Figure 3.

Inhibition of EST2 by diethyl (4-nitrophenyl) phosphate (paraoxon). Description of the kinetic (A) and molecular (B) mechanism of the inhibition of EST2 by paraoxon. The inhibited enzyme is frozen in a stable covalent intermediate with the organophosphate group of pesticide. (C) Plot of EST2 residual activity in presence of an increasing concentration of paraoxon. In the insert, the percentage of the inhibition of EST2 in the presence of this increasing concentration of paraoxon. The error bars represent the standard deviation (SD) from three independent experiments.