Table 2.
Sample Name | Charcoal Rot Disease | Soybean Tissue Type | (-)-Botryodiplodin (µg/g) a | Phomenone (µg/g) a | Gigantenone (µg/g) a | Phaseolinone (µg/g) a |
---|---|---|---|---|---|---|
RTS 999 302 | Yes | Roots | 0.786 | 0 | 0 | 0 |
USG 7582 306 | Yes | Roots | 0.13 | 0 | 0 | 0 |
DK B58-51 326 | Yes | Roots | 0.23 | 0 | 0 | 0 |
AG 5903 324 | Yes | Roots | 0.046 | 0 | 0 | 0 |
AG 5701 329 | Yes | Roots | 0.139 | 0 | 0 | 0 |
PGX 5703 313 | Yes | Roots | 0.334 | 0 | 0 | 0 |
PGY 5822 319 | Yes | Roots | 0.332 | 0 | 0 | 0 |
ESXVT-46 328 | Yes | Roots | 0.134 | 0 | 0 | 0 |
P 95 B96 301 | Yes | Roots | 0.006 | 0 | 0 | 0 |
P GX 5714 311 | Yes | Roots | 0.141 | 0 | 0 | 0 |
Garst 5812 331 | Yes | Roots | 0.209 | 0 | 0 | 0 |
DK 5767 32 | Yes | Roots | 0.061 | 0 | 0 | 0 |
All samples tested | Yes | Seeds | 0 | 0 | 0 | 0 |
All samples tested | Yes | Pulp | 0 | 0 | 0 | 0 |
All samples tested | Yes | Branches | 0 | 0 | 0 | 0 |
All samples tested | Yes | Twigs | 0 | 0 | 0 | 0 |
All samples tested | Yes | Leaves | 0 | 0 | 0 | 0 |
Undiseased control b | No | Roots | 0 | 0 | 0 | 0 |
a Identification and quantification of toxins in samples by LC/MS were based on one standard due to the limited amount of standards available. b Soybean plants of the Saline cultivar with no detectable sign of charcoal rot disease were collected from commercial fields in Mississippi. Samples of the same six tissues were taken, pooled, and extracted in the same way as tissues from diseased plants and the extracts were assayed by LC/MS in the same manner. Extracts of all undiseased soybean tissues, including roots, contained no detectable (-)-botryodiplodin or other toxin.