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. 2019 Oct 25;11(11):618. doi: 10.3390/toxins11110618

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) CagA immunoreactivity in the basal cytoplasm of an epithelial cell showing intense immunogold deposition over clear, irregularly shaped proteasome particle-rich cytoplasmic structure (PaCS) areas. Note CagA reactivity of minute PaCSs arising focally in the cytosol interposed between PaCS-surrounding ribosomes. Also note sparse minute CagA deposits (arrowheads) in the cytosol adjacent to the rough endoplasmic reticulum or free ribosomes. n, epithelial cell nucleus; lp, lamina propria. (B,C) Double CagA (smaller gold particles) and 19S proteasome (larger gold particles) immunolabelling identifies the clear, cytoskeleton-poor PaCS as a CagA- as well as proteasome-rich structure. Note that PaCS-surrounding ribosomes in (B) and on top of (C) differ in electron density and shape from the proteasome-reactive barrel-like particles filling the PaCSs. r, ribosomes. (D) The barrel-like shape and thinly punctate high-resolution pattern of 20S proteasome-reactive particles (5-nm gold particles) is recognized when under favorable cutting plan. Sparse CagA (15-nm gold particles) and polyubiquitinated proteins (10-nm gold particles) immunoreactivities are also visible.