Table 2.

Pharmacological features of Site 4 interacting spider knottins resulting in reduction of peak inward current.

Features	Examples
Depolarizing shift in voltage-dependence of activation	ProTx-I [103], ProTx-II [77,103,104], JzTx-III [105,106], CcoTx-I [73], CcoTx-2 [73], CcoTx-3 [73], PaurTx-3 [73], JzTx-V [79,107], JzTx-IX [108], Hm-3 [109], Cd1a [110], Pre1a [111], Pn3a [61], Df1a [92], JxTx-XI [91], JzTx-35 [112]
No effect in voltage-dependence of activation	HwTx-IV [104,113,114,115], HnTx-III [116,117], JzTx-34 [118,119], Hm-1 [120], Hm-2 [120], Hd1a [121], GpTx-1 [122], Hl1a [123], PnTx1 [124], ProTx-III [80], Pre1a [111], JzTx-14 [125]
Hyperpolarizing shift in voltage-dependence of steady-state inactivation	HnTx-III [116], HnTx-IV [116], JzTx-V [79], Hm-1 [120], Hm-2 [120], JzTx-35 [112], PnTx4 (5-5) [126], Df1a [92], JzTx-34 [119]
Delay in channel inactivation	ProTx-II [104], JzTx-XI [91], Df1a [92], JzTx-14 [125]
Decreased channel recovery from inactivation	HnTx-III [116], JzTx-XI [91], Pn3a [61]
No effect in channel recovery from inactivation	HnTx-IV [116], JzTx-34 [118], HnTx-III [127], Hd1a [121]
Hyperpolarizing shift in voltage-dependence of activation	Df1a [92]
Depolarizing shift in voltage-dependence of steady-state inactivation	Df1a [92]