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. 2019 Nov 14;24(22):4119. doi: 10.3390/molecules24224119

Figure 6.

Figure 6

CS-N inhibits EMT and ECM accumulation in HK-2 cells via p38 and ERK MAPK pathway. (A) HK-2 cells were pretreated with inhibitors of p38 MAPK (SB203580, 50 μM) for 30 min, followed by the treatment with CS-N (100 μg/mL) for 48 h. Expression levels of p38, P-p38, E-cadherin, and collagen I were analyzed by western blot. (B) HK-2 cells were pretreated with inhibitors of ERK (U0126, 20 μM) for 30 min, followed by the treatment with CS-N (100 μg/mL) for 48 h. Expression levels of ERK, P-ERK, E-cadherin, and collagen I were analyzed by western blot. Data are presented as the means ± SDs, triplicate. ** p < 0.01; *** p < 0.001 compared with NG group; # p < 0.05; ## p < 0.01; ### p < 0.001 compared with HG group; & p < 0.05; && p < 0.01 compared with p38 or ERK inhibitor plus CS-N.