Figure 4.

Effects of the ethyl acetate (EtOAc) subfractions (EA1-7) on cell viability (A) and NO production (B). RAW 264.7 cells were treated with EtOAc subfractions (100 µg/mL). Cell viability and the amount of nitrite in the culture medium were measured by MTT assay and Griess reagent, respectively. Nitrite concentrations of non-treated and LPS-treated controls were 0.6 ± 0.1 μM and 16.5 ± 0.2 μM, respectively. The data are expressed as the mean ± SD (n = 3) of three individual experiments, and * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with LPS-treated controls.