Table 5.
Retention time and mass spectrometry characteristics for carotenoids tentatively identified in the four microalgae species investigated.
| Species | Peaks N⁰ | Rt(min) | ʎ max(nm) | m/zAPCI+/MS | Compound Identification | Sample State |
|---|---|---|---|---|---|---|
| Phaeodactylum tricornutum | 1 | 3.26 | 423, 666 | 545.3 | Unidentified carotenoids | d.bio |
| 2 | 3.48 | 331, 361, 422 | 556.4 | Unidentified carotenoids | d.bio | |
| 3 | 5.77 | 446 | 659.9 | All-E-Fucoxanthin | d.bio, c.liq | |
| 4 | 6.38 | 333, 442 | 659.9 | Fucoxanthin isomer | c.liq | |
| 5 | 7.96 | 436 | 659.9 | Fucoxanthin type | d.bio | |
| 6 | 9.54 | 441 | 659.9 | Fucoxanthin type | d.bio | |
| 7 | 12.93 | 422,446,476 | 585.9 | Diadinoxanthin | d.bio, c.liq | |
| 8 | 54.61 | 425, 450, 478 | 537.9 | beta-carotene | c.liq | |
| 9 | 7.55 | 417, 442, 471 | 601.3 | Vaucheriaxanthin | d.bio, c.liq | |
| 10 | 12.01 | 330, 436, 463 | 569.9 | cis-15-lutein | c.liq | |
| 11 | 13.21 | 331,465 | 569.9 | cis-13-lutein | c.liq | |
| 12 | 14.75 | 442, 472 | 569.9 | Lutein | d.bio, c.liq | |
| 13 | 20.33 | 461 | 551.9 | Echinone | d.bio | |
| 8 | 54,59 | 425, 450, 478 | 537.9 | beta-carotene | d.bio, c.liq | |
| 14 | 55.38 | 342, 424, 446 | 537.9 | 9-cis-beta-carotene | d.bio | |
| Tetraselmis suecica | 15 | 5.76 | 449, 467 | 659.9 | Fucoxanthin | d.bio, c.liq |
| 16 | 10.60 | 253, 345, 457, 592, 640 | 601.9 | Violaxanthin | d.bio, c.liq | |
| 17 | 11.11 | 345, 457, 592, 640 | 600.8 | Cis- Prasinoxanthine | d.bio | |
| 12 | 14.47 | 442, 472 | 569.9 | Lutein | d.bio, c.liq | |
| 8 | 54.59 | 425, 450, 478 | 537.9 | beta-carotene | c.liq | |
| Nannochloropsis gaditana | 18 | 5.27 | 418, 438, 465 | 601.9 | Neoxanthin | d.bio |
| 19 | 9.92 | 422, 444, 472 | 585.9 | Antheraxanthin | d.bio | |
| 20 | 10.75 | 427, 449, 477 | 569.9 | Zeaxanthin | d.bio, c.liq | |
| 8 | 54.59 | 425, 450, 478 | 537.9 | beta-carotene | c.liq |
d.bio: Dry biomass; c.liq: Crude liquid.