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. 2019 Nov 13;24(22):4100. doi: 10.3390/molecules24224100

Figure 2.

Figure 2

Figure 2

Effects of dicentrine on TNF-α-induced A549 cells apoptosis in a caspase-dependent manner and the expression of antiapoptotic proteins. A549 cells were pretreated with various concentrations of dicentrine for 4 h and then cotreated with 25 ng/mL of TNF-α to 24 h. After the combined treatment with dicentrine (0–40 μM) and TNF-α, the whole cell extracts were prepared and analyzed by the western blot analysis to detect the expression of caspase-8, -9, -3, and poly(ADP-ribose) polymerase (PARP) (A). The complex between procaspase-8 and receptor-interacting protein (RIP) was determined by coimmunoprecipitation after incubating with dicentrine (25 μM) for 4 h and then cotreatment with 25 ng/mL of TNF-α for 12 h (B). The levels of antiapoptotic proteins such as cFLIPs, cIAP-2, and Bcl-XL was measure after cotreated with dicentrine (0–20 μM) and TNF-α. (C,D). The data are represented as mean ± S.D. ** indicates p < 0.01, when compared to those treated with TNF-α alone.