Figure 3.
Effects of dicentrine on TNF-α-induced invasion and migration. The matrix gel was coated on membrane filters for invasion assays (A), and the gelatin was coated for migration assays (B). A549 cells with the number of 1.25 × 105 cells were cultures in the upper chamber and incubated with various concentrations of dicentrine (0–20 μM) in a Dulbecco’s modified Eagle’s medium (DMEM)-free medium, and the lower chamber was filled with 25 ng/mL of TNF-α. After 24 h of incubation, the cells that actively migrated to the lower surface of the filter were determined. The data are represented as mean ± S.D. of three independent experiments. Sample groups were significantly different from the TNF-α-treated group (* indicates p < 0.05, and ** indicates p < 0.01).