Figure 4.

Dicentrine suppresses TNF-α-induced expression of metastatic protein. A549 cells were pretreated with various concentrations of dicentrine (0–20 μM) for 4 h and then cotreatment with 25 ng/mL of TNF-α for 24 h. The whole cell extracts were prepared and analyzed by the western blot analysis using antibodies against cell metastatic proteins (MT1-MMP, uPAR, ICAM-1, and Cox-2) (A,B). The culture supernatants of the treated cells were collected, and the secretions of MMP-9 were analyzed by gelatin zymography (C,D). The data are represented as mean ± S.D. * indicates p < 0.05, and ** indicates p < 0.01, when compared to those treated with TNF-α alone.