Table 1.
The detailed information of identified components in positive ion mode and negative ion mode byUHPLC-ESI-Q-TOF-MS/MS.
| No. | Rt | m/z | CAS&Pubchem ID | Identification | Adducts | Formula | Error (ppm) | p-Value |
|---|---|---|---|---|---|---|---|---|
| 1 | 1.01 | 132.0541 | 3130-87-8 | Asparagine | M − H2O − H, M − H | C4H8N2O3 | 4.74 | 3.7 × 10−4 |
| 2 | 1.05 | 195.0513 | 70849-23-9 | 2,3,4,5-tetrahydroxypentanal | M + FA − H | C5H10O5 | 2.15 | 2.0 × 10−5 |
| 3 | 1.10 | 342.1158 | 57-50-1 | Sucrose | M − H, M + FA − H | C12H22O11 | −1.09 | 1.7 × 10−4 |
| 4 | 7.43 | 203.0829 | 73-22-3 | L-Tryptophan | M − H | C11H12N2O2 | 1.58 | 6.1 × 10−3 |
| 5 | 10.23 | 161.0249 | 93-35-6 | 7-Hydroxycoumarin | M − H | C9H6O3 | 3.09 | 3.4 × 10−10 |
| 6 | 11.37 | 209.0460 | 25429-38-3 | Coumaric Acid | M + FA − H | C9H8O3 | 3.00 | 7.7 × 10−5 |
| 7 | 11.72 | 431.0987 | 152-95-4 | Sophoricoside | M−H | C21H20O10 | 0.77 | 1.5 × 10−6 |
| 8 | 12.88 | 283.0608 | 20575-57-9 | Calycosin | M − H | C16H12O5 | −1.52 | 2.5 × 10−3 |
| 9 | 12.91 | 447.1312 | 464196-55-2 | Licoagroside D | M − H | C22H24O10 | 3.46 | 4.2 × 10−3 |
| 10 | 13.02 | 187.0979 | 66923-62-4 | Azelaic acid | M − H | C9H16O4 | 1.61 | 2.9 × 10−3 |
| 11 | 13.40 | 299.0560 | 1447-88-7 | Hispidulin | M − H | C16H12O6 | −0.47 | 1.8 × 10−5 |
| 12 | 13.76 | 255.0663 | 961-29-5 | Isoliquiritigenin | M − H | C15H12O4 | 0.07 | 2.3 × 10−2 |
| 13 | 13.76 | 463.1618 | 94367-43-8 | Isomucronulatol 7-O-glucoside | M − H | C23H28O10 | 1.71 | 3.3 × 10−6 |
| 14 | 13.82 | 267.0658 | 485-72-3 | Formononetin | M − H | C16H12O4 | −1.66 | 9.1 × 10−09 |
| 15 | 14.09 | 971.4902 | 147540-80-5 | Sophoraflavoside II | M − H | C48H76O20 | 4.61 | 1.2 × 10−3 |
| 16 | 14.42 | 271.0614 | 25515-46-2 | Naringenin chalcone | M − H | C15H12O5 | 0.63 | 9.0 × 10−4 |
| 17 | 14.42 | 831.4781 | 170969-74-1 | Cyclocanthoside E | M + FA − H | C41H70O14 | 4.21 | 4.1 × 10−4 |
| 18 | 14.85 | 827.4408 | 1037218-20-4 | Eremophiloside J | M + FA − H | C41H66O14 | −1.67 | 1.5 × 10−6 |
| 19 | 14.89 | 784.4650 | 84687-43-4 | Astragaloside IV | M − H, M + FA − H | C41H68O14 | 4.28 | 2.6 × 10−8 |
| 20 | 15.17 | 942.5210 | 51330-27-9 | Soyasaponin I | M − H, M + FA − H | C48H78O18 | 2.30 | 1.3 × 10−5 |
| 21 | 15.25 | 871.4705 | 84676-89-1 | Astragaloside II | M + FA − H | C43H70O15 | 0.96 | 6.0 × 10−4 |
| 22 | 15.35 | 301.1075 | 20878-97-1 | (−)-Mucronulatol | M − H | C17H18O5 | −2.23 | 4.2 × 10−4 |
| 23 | 15.51 | 915.4903 | 156769-94-7 | Agroastragaloside I | M + FA − H | C45H74O16 | −4.90 | 1.9 × 10−4 |
| 24 | 15.60 | 909.4863 | 386273-42-3 | Trojanoside I | M − H | C47H74O17 | 1.04 | 3.0 × 10−9 |
| 25 | 16.10 | 868.4847 | 84680-75-1 | Astragaloside I | M + Cl, M + FA − H | C45H72O16 | 3.08 | 1.8 × 10−4 |
| 26 | 17.59 | 296.2344 | 80286-58-4 | Artemisic Acid | M − H2O − H, M − H, M + Cl | C18H32O3 | −2.37 | 6.9 × 10−4 |
| 27 | 1.08 | 543.1320 | 439336 | Galactomannan | M + K | C18H32O16 | −0.30 | 4.6 × 10−2 |
| 28 | 1.39 | 158.1173 | 108866-42-8 | 2-Epilentiginosine | M + H | C8H15NO2 | −1.69 | 1.6 × 10−7 |
| 29 | 12.41 | 446.1217 | 20633-67-4 | Calycosin-7-O-Beta-d-Glucoside | M + H, M + Na | C22H22O10 | 0.97 | 1.6 × 10−3 |
| 30 | 12.61 | 477.1388 | 113235-89-5 | 7,2′-Dihydroxy-3′,4′-dimethoxyisoflavone 7-O-glucoside | M + H | C23H24O11 | −0.78 | 5.3 × 10−15 |
| 31 | 13.47 | 563.1392 | 27661-51-4 | Leucoside | M + H − H2O | C26H28O15 | −0.57 | 8.2 × 10−17 |
| 32 | 14.07 | 430.1267 | 486-62-4 | Ononin | M + H, M + Na | C22H22O9 | 0.73 | 4.3 × 10−7 |
| 33 | 14.12 | 287.0911 | 122587-87-5 | (3R)-3′,8-Dihydroxyvestitol | M + H − H2O | C16H16O6 | −0.98 | 6.9 × 10−13 |
| 34 | 14.27 | 316.0946 | 158991-20-9 | Astragaluquinone | M + H, M + Na | C17H16O6 | −0.26 | 3.9 × 10−8 |
| 35 | 14.41 | 462.1519 | 94367-42-7 | Methylnissolin-3-O-glucoside | M + H, M + NH4, M + Na | C23H26O10 | −1.59 | 9.2 × 10−7 |
| 36 | 14.44 | 300.0988 | 72026-91-6 | Astraciceran | M + H − H2O, M + | C17H16O5 | −3.15 | 1.7 × 10−11 |
| 37 | 15.00 | 314.0791 | 3301-49-3 | Kumatakenin | M + H, M + Na, M + K | C17H14O6 | 0.18 | 1.9 × 10−12 |
| 38 | 15.22 | 472.3549 | 465-99-6 | Hederagenin | M + H − H2O, M + H | C30H48O4 | −0.67 | 2.2 × 10−11 |
| 39 | 15.24 | 302.1150 | 158784-72-6 | 8-Methoxyvestitol | M + H, M + Na | C17H18O5 | −1.51 | 1.3 × 10−6 |
| 40 | 15.49 | 316.0946 | 69359-09-7 | Pendulone | M + H−H2O, M H, M + Na | C17H16O6 | −0.24 | 8.9 × 10−4 |
| 41 | 15.86 | 490.3650 | 78574-94-4 | Cycloastragenol | M + H − H2O, M + Na | C30H50O5 | −1.68 | 4.5 × 10−10 |
| 42 | 15.88 | 807.4503 | 84687-42-3 | Astragaloside III | M + Na | C41H68O14 | 0.18 | 1.9 × 10−9 |
| 43 | 16.20 | 298.0842 | 740-33-0 | Mosloflavone | M + H, M + Na | C17H14O5 | 0.34 | 4.2 × 10−14 |
| 44 | 16.22 | 274.2743 | 57-10-3 | hexadecanoic acid | M + NH4 | C16H32O2 | 1.05 | 2.4 × 10−2 |
| 45 | 16.22 | 301.1067 | 73340-41-7 | 3-Hydroxy-9,10-dimethoxyptercarpan | M + H | C17H16O5 | −1.09 | 1.2 × 10−6 |
| 46 | 16.41 | 645.3974 | 101683491 | Trigonoside I | M + Na | C35H58O9 | 0.12 | 2.2 × 10−7 |
| 47 | 16.44 | 620.3922 | 13943265 | Cycloorbigenin 3-O-beta-d-xylopyranoside | M + H − H2O, M + Na | C35H56O9 | −0.37 | 8.5 × 10−7 |
| 48 | 17.03 | 473.3617 | 86541-79-9 | Astragenol | M + H − H2O | C30H50O5 | −1.66 | 4.0 × 10−5 |
| 49 | 17.07 | 687.4079 | 133550435 | Cyclogaleginoside A | M + Na | C37H60O10 | 0.11 | 4.0 × 10−8 |
| 50 | 18.42 | 279.2315 | 463-40-1 | Linolenic acid | M + H | C18H30O2 | −1.36 | 7.0 × 10−5 |
| 51 | 19.93 | 263.2367 | 693-77-6 | Octadeca-9,12-dienoic acid | M + H − H2O | C18H32O2 | −0.73 | 5.9 × 10−3 |
| 52 | 21.68 | 409.3823 | 545-47-1 | Lupeol | M + H − H2O | C30H50O | −1.45 | 2.1 × 10−2 |
| 53 | 21.93 | 397.3826 | 83-46-5 | (−)-beta-Sitosterol | M + H − H2O | C29H50O | −0.76 | 1.8 × 10−2 |