Table 1.
Strains and plasmids used in this study.
| Strain | Characteristics | Reference |
|---|---|---|
| C. glutamicum strains | ||
| WT | Wild type, ATCC 13032 | [40] |
| MB001 | prophage cured, genome reduced ATCC 13032 | [39] |
| BETA4 | MB001 derivative with deletion of crtYEb (cg0717-0719) and crtR (cg0725) and integration of Ptuf-dxs, Ptuf-crtEBI, Ptuf-crtYPa | [13] |
| ASTA1 | BETA4 derivative with pSH1-crtWFp and pECXT99A-crtZFp | [13] |
| ASTA* | BETA4 derivative with pSH1-crtZ~WFp | This work |
| Other strains | ||
| E. coli DH5α | F-thi-1 endA1 hsdr17(r-, m-) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 | [41] |
| Pantoea ananatis | Wild type, ATCC 33244, DSM 17873, Z96081 | [42] |
| Fulvimarina pelagi | Wild type, ATCC BAA-666, DSM 15513, AY178860 | [43] |
| Plasmids | ||
| pECXT99A (pECXT) | TetR, PtrclacIq, pGA1 oriVCg, C. glutamicum/E. coli expression shuttle vector | [44] |
| pECXT-idi | pECXT99A derivative for IPTG-inducible expression of idi (cg2531) | This work |
| pECXT-idsA | pECXT99A derivative for IPTG-inducible expression of idsA (cg2384) | This work |
| pECXT- crtBI | pECXT99A derivative for IPTG-inducible expression of crtBI (cg0721/0720) | This work |
| pECXT-idi-idsA | pECXT99A derivative for IPTG-inducible expression of idi (cg2531) and idsA (cg2384) | This work |
| pECXT-idsA-crtBI | pECXT99A derivative for IPTG-inducible expression of idsA (cg2384) and crtBI (cg0721/0720) | This work |
| pECXT-idi-idsA-crtBI | pECXT99A derivative for IPTG-inducible expression of idi (cg2531), idsA (cg2384) and crtBI (cg0721/0720) | This work |
| pSH1 | KmR, Ptuf, pHM519 oriVCg, C. glutamicum/E. coli expression shuttle vector | [13] |
| pSH1-crtW-crtZ | pSH1 derivative for constitutive expression of the artificial operon comprising crtW and crtZ from F. pelagi | This work |
| pSH1-crtW~Z | pSH1 derivative for constitutive expression of crtW~Z encoding for a fusion protein comprising CrtW and CrtZ from F. pelagi | This work |
| pSH1-crtZ-crtW | pSH1 derivative for constitutive expression of the artificial operon comprising crtZ and crtW from F. pelagi | This work |
| pSH1-crtZ~W | pSH1 derivative for constitutive expression of crtZ~W encoding for a fusion protein comprising CrtZ and CrtW from F. pelagi | This work |