Table 4.
Primers used to detect cyanobacteria and gene clusters of the cyanotoxins microcystin, saxitoxin, anatoxin, and cylindrospermopsin.
| Gene | Primer | Primer Sequence 5’-3’ | Size (bp) | Reference | Positive Control |
|---|---|---|---|---|---|
| 16S | CYA106F/CYA781R | CGGACGGGTGAGTAACGCGTGA GACTACTGGGGTATCTAATCCCATT |
675 | [81] | - |
| 16S | CYA359F/1494R | GGGGAATYTTCCGCAATGGG TACGGCTACCTTGTTACGAC |
1135 | [81,82] | - |
| mcyA | CD1F/CD1R | AAAATTAAAAGCCGTATCAAA AAAAGTGTTTTATTAGCGGCTCAT |
297 | [83] | LEGE91339-Microcystis aeruginosa |
| mcyE | HEPF/HEPR | TTTGGGGTTAACTTTTTTGGGCATAGTC AATTCTTGAGGCTGTAAATCGGGTTT |
472 | [40] | LEGE91339-Microcystis aeruginosa |
| sxtI | SXTI 682F/SXTI 877R | GGATCTCAAAGAAGATGGCA GCCAAACGCAGTACCACTT |
100 | [84] | LMECYA 040-Aphanizomenon gracile |
| anaC | anaCF/anaCR | TCTGGTATTCAGTCCCCTCTAT CCCAATAGCCTGTCATCAA |
366 | [85] | LEGE X-002-Anabaena sp. |
| pks | M4/M5 | GAAGCTCTGGAATCCGGTAA AATCCTTACGGGATCCGGTGC |
535/ 540 |
[86] | LEGE 97,047-Cylindrospermopsis raciborskii |
| ps | M13/ M14 |
GGCAAATTGTGATAGCCACGAGC GATGGAACATCGCTCACTGGTG |
511/ 534 |
[86] | LEGE 97,047-Cylindrospermopsis raciborskii |