Table 1.
Linearity, LOD, and LOQ of the standard compounds.
| Compounds | tR (min) |
Equation (Linear Model)a |
Linear Range (mg/mL) |
r2 b | LOD c (mg/mL) |
LOQ d (mg/mL) |
|---|---|---|---|---|---|---|
| 1 | 12.4 | y = 3,442,356x − 8492 | 0.02–4 | 0.9992 | 0.0009 | 0.0026 |
| 2 | 13.5 | y = 1,353,127x + 13,963 | 0.02–4 | 0.9998 | 0.0022 | 0.0065 |
| 3 | 22.9 | y = 1,269,657x – 23,913 | 0.02–4 | 0.9995 | 0.0007 | 0.0020 |
| 4 | 56.3 | y = 1,528,845x + 2521 | 0.03–0.7 | 0.9990 | 0.0132 | 0.0400 |
| 5 | 57.2 | y = 1,462,227x – 826 | 0.01–0.3 | 0.9999 | 0.0027 | 0.0081 |
a y: peak area at 200 nm; x: standard concentration (mg/mL). b r2: coefficient of determination with 7–12 indicated points in the calibration curves. c LOD: limit of detection; S/N = 3 (n = 5). d LOQ: limit of quantification; S/N = 10 (n = 5).