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. 2019 Nov 6;17(11):632. doi: 10.3390/md17110632

Table 1.

Real-time PCR primers of immune defense-related genes in Drosophila.

Gene Name Sequence 5′-3′ Annealing Temp
Duox F:CAGTTTCGAACGAACTCTTGG
R:CATGGTTTTCCAATTATGCAGATTC
54 °C
IMD F:AAAACGGTCAGCATTGTGGC
R:GTCCTTCCGACATGCCAAGA
52 °C
Drosocin F:GCACAATGAAGTTCACCATCGT
R:CCACACCCATGGCAAAAAC
56 °C
PIMS F:ATCGTTTTCCTGCTGCTTGC
R:ATTACTTGCAGTTGCCCGGA
59 °C
Rp49 F:AGGGTATCGACAACAGAGTG
R:CACCAGGAACTTCTTGAATC
52 °C