Table 1.
Serial dilutions of aminoglycosides performed in two 96-well plates (plate A and B).
| Equiv. (plate A) |
Vol (μL) | Buffer vol (μL) |
SM conc. (μM) |
Equiv. (plate B) |
Vol (μL) | Buffer vol (μL) |
SM conc. (μM) |
|---|---|---|---|---|---|---|---|
| 16 | 0.96 | 299.04 | 6.4 | 20 | 1.2 | 298.8 | 8 |
| 10 | 187.50 | 112.50 | 4 | 14 | 210.00 | 90.00 | 5.6 |
| 7 | 210.00 | 90.00 | 2.8 | 8 | 171.43 | 128.57 | 3.2 |
| 5 | 214.29 | 85.71 | 2 | 6 | 225.00 | 75.00 | 2.4 |
| 3 | 180.00 | 120.00 | 1.2 | 4 | 200.00 | 100.00 | 1.6 |
| 2 | 200.00 | 100.00 | 0.8 | 2.5 | 187.50 | 112.50 | 1 |
| 1 | 150.00 | 150.00 | 0.4 | 1.5 | 180.00 | 120.00 | 0.6 |
| 0 | 0.00 | 300.00 | 0 | 0.5 | 100.00 | 200.00 | 0.2 |
The volumes listed in the first row indicate the amount of the 2mM stock solution to be diluted with buffer. The volumes in the following rows indicate the amount of the solution from the well above to be diluted with buffer to obtain the desired small molecule (SM) concentration.