Figure 2.

Split BS2 can detect small-molecule-activated PPIs. (a) HEK293T cells cotransfected with BS2_N-fused FRB and FKBP-fused BS2_C or HEK293T control cells (white) were incubated with rapamycin (green) or a DMSO control (gray). After 24 h, fluorescein-CM₂ was applied, and the cells were analyzed by a plate reader for fluorescence. (b) HEK293T cells were treated identically to conditions in part a and analyzed by fluorescence microscopy. Error bars are the standard deviation for n = 4 replicates. Unpaired t test; ***P < 0.0001. Scale bars shown are 20 μm.