Table 1. Electrostatic Components for Proteins and AcVES3.
| protein | basic A.A. in ID | calc pI |
|---|---|---|
| EGFP | 5.6 | |
| For EGFP/mRuby3, N-Terminal IDn = G(RH)nR(GGSGS)2- | ||
| ID1-EGFP | 2 Arg, 1 His | 5.9 |
| ID2-EGFP | 3 Arg, 2 His | 6.1 |
| ID3-EGFP | 4 Arg, 3 His | 6.3 |
| ID4-EGFP | 5 Arg, 4 His | 6.5 |
| ID5-EGFP | 6 Arg, 5 His | 6.7 |
| ID6-EGFP | 7 Arg, 6 His | 7.0 |
| H6-EGFP | 6 His | 6.0 |
| H12-EGFP | 12 His | 6.2 |
| NH6-EGFP | 3 Arg, 2 Lys, 6 His | 6.3 |
| mRuby3 | 5.8 | |
| ID5-mRuby3 | 6 Arg, 5 His | 8.4 |
| For IFNα, C-Terminal IDn = -(SGSGG)2(RH)nR | ||
| IFNα | 5.4 | |
| IFNα-ID3 | 4 Arg, 3 His | 7.0 |
| IFNα-ID5 | 6 Arg, 5 His | 8.4 |
| AcVES3 peptide hydrogel | net charge | |
|---|---|---|
| Ac-VEVSVSVEVDPLPTEVSVEVEV-NH2 | –5 | |