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. 2019 Dec 3;19:1180. doi: 10.1186/s12885-019-6333-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The IL-6/pSTAT3/HIC1 axis in MDA-MB-231 cells. ELISA results of secreted IL-6 and expression of pSTAT3 and HIC1 in the BrCA cell lines. b Real-time PCR was used to examine the IL-6 knockdown effect in MDA-MB-231 cells. c ELISA results to confirm the decreased expression of IL-6 in 231-shIL-6 cells. d HIC1 expression was increased in sh-IL-6 cells. e Cell viability assay in 231-shIL-6 cells for 2, 4 and 6 days. f Colony formation was decreased in 231-shIL-6 cells after 10 days. g Examination of the cell cycle by flow cytometry analysis in 231-shIL-6 cells