Table 1.

Recommended environmental data to collect alongside eDNA samples.

priority	variable	reference
recommended	date (season)	[112]
	water temperature	[30–36]
	flow velocity and/or discharge	[7,71]
	water body width and depth	[7,35,71]
	salinity	[38]
suggested	pH	[24,34,35]
	turbidity	[1,37]
	microbial growth (as chlorophyll a or organic matter)	[18,26]
	substrate type	[8,105]
	nutrient levels	[105]
	geomorphological features (stream slope, average stream-scale form, longitudinal roughness, etc.)	[73]