Fig. 6.

Plasma EV integrin β1 predicted SACC lung metastasis in mice. a Schematic of the animal experiments using BALB/c nude mice. b POSTN expression in the lungs of mice at week 3. Metastatic colony number and area in the lungs of mice at week 5. Survival analysis for the mice raised for up to 12 weeks. c Western blot analysis of plasma EV integrin β1 expression in the mice at the pre-metastatic stage. d Western blot analysis of plasma EV integrin β1 expression in the mice at the metastatic stage. e Illustration of the CAF EVs constructed pre-metastatic niche in the lungs. Scale bar = 50 μm. * P < 0.05, ** P < 0.01