Fig. 7.

MORC2 dimerization is implicated in altered nucleosome stability after DNA damage and subsequent DDR signaling. a HA-MORC2 and HA-MORC2 ∆C82 in pCDH-CMV-MCS-EF1-copGFP expression vector were re-expressed in MORC2 KO HeLa cells by lentiviral infection. After 48 h of infection, GFP-positive cells were selected by FACS. Expression levels of MORC2 in established stable cell lines were verified by immunoblotting analysis. b HeLa cells stably expressing HA-MORC2 and HA-MORC2 ∆C82 were treated with 8 μM CPT for 1 h and subjected to salt solubilization assays as described above. Immunoblotting analysis was carried out with the indicated antibodies. NPM and hnRNPM were used as loading controls. c-d HeLa cells stably expressing HA-MORC2 and HA-MORC2 ∆C82 were treated with 8 μM CPT for 1 h. Immunofluorescent staining was carried out using the indicated antibodies (c). Quantitative results (foci per cell) are shown in d. Eight representative cells stained with the individual antibody were counted. e HeLa cells stably expressing HA-MORC2 and HA-MORC2 ∆C82 were treated with CPT at the indicated doses and subjected to clonogenic survival assays. **, p < 0.01