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. 2019 Oct;189(10):1916–1932. doi: 10.1016/j.ajpath.2019.07.004

Figure 2.

Figure 2

Analysis of KHSRP expression in primary tissue samples reveals a tumor-specific overexpression with prognostic value. A: Western blot analysis of KHSRP in lysates from fresh-frozen tissue samples of patients with stage IV colorectal cancer (CRC), comparing tumor (T) with matched normal (N) tissue from each patient. A representative blot for two patients is shown along with semiquantitative densitometric analysis of all patients (the box-and-whisker plot depicts interquartile range, median, minimum, and maximum limits). A t-test was performed. B: Representative images from tissue microarray (TMA) sections of normal and CRC tissue stained for KHSRP. C: Quantitation of stromal and epithelial KHSRP staining in normal colon versus primary CRC tissue samples from the TMA. Individual points are shown. A Wilcoxon test was performed. D: T/N ratios in the epithelium were used to segregate patients, using 20% as a cutoff (T/N < 1.2 versus T/N > 1.2). Survival curves were calculated, according to the Kaplan-Meier method, and compared using the Cox-Mantel log-rank test. The hazard ratio (HR) and associated CI are shown. Data are expressed as means ± SD (C). n = 9 (A). **P < 0.01, ***P < 0.001, and ****P < 0.0001. Scale bars: 200 μm (B, left column); 100 μm (B, right column). Original magnification: ×10 (B, left column); ×20 (B, right column).