Figure 6.

KHSRP regulates expression of key proteins that modulate the tumorigenic microenvironment. Differential expression of secreted proteins was analyzed by shotgun proteomics in conditioned media from SW620 cells treated with doxycycline for 4 days to induce shRNA-mediated KHSRP knockdown. A: Volcano plot showing differentially regulated proteins in the doxycycline-treated samples compared with control, colored in red (log₂ fold change > |1|; P < 0.05). Proteins that are differentially regulated above the fold change threshold with a nonsignificant P value are colored in yellow. Dashed lines indicate the thresholds on both axes. B: Dot plot of results from a Gene Ontology (GO) analysis of the 40 highly differentially regulated proteins. GO terms are reported with their category and plotted by gene ratio (the number of genes in one GO term compared with the total). Dots are sized in proportion to the number of hits within a GO term and colored by P value. C: Label-free quantitation of relative abundance of the indicated five proteins in the doxycycline-treated versus control samples. D: Western blot analysis of Ephrin B2 and S100 calcium-binding protein A11 (S100-A11) in conditioned media from control or doxycycline-treated SW620 cells. A Coomassie stain is shown for protein loading control. ****P < 0.0001 (t-test). BP, biological process; CC, cellular component; KP, Kegg pathway; MF, molecular function; RhoGDIα, Ras homolog family–specific guanine nucleotide dissociation inhibitor–α; UP, uniprot annotation.