Table 1.
Antibodies Used for Flow Cytometry and Immunofluorescence
| Antigen | Vendor | Catalog no. | Species specificity | Clone name | Concentration, μg/mL |
|
|---|---|---|---|---|---|---|
| IF | Flow cytometry | |||||
| CD3 | Abcam (Cambridge, UK) | ab5690 | Rabbit anti-human (P) | N/A | 2 | N/A |
| CD4 | Abcam | ab133616 | Rabbit anti-human (P) | N/A | 1.4 | N/A |
| CD8 | Santa Cruz Biotechnology (Dallas, TX) | sc-7188 | Rabbit anti-human (P) | N/A | 2 | N/A |
| CD11b | R&D Systems (Minneapolis, MN) | MAB16992 | Mouse anti-human (M) | 238439 | 5 | N/A |
| CD11b | BioXCell (West Lebanon, NH) | BE0007 | Rat anti-mouse (M) | M1/70 | 10 | N/A |
| CD14 | Santa Cruz Biotechnology | sc-1182 | Mouse anti-human (M) | UCH-M1 | 2 | N/A |
| CD18 | Invitrogen (Carlsbad, CA) | MA1810 | Mouse anti-human (M) | TS1/18.1.2.11.4 | 10 | N/A |
| CD38 | R&D Systems | MAB24041 | Mouse anti-human (M) | 240726 | 5 | N/A |
| CD56 | Santa Cruz Biotechnology | sc-106 | Mouse anti-human (M) | ERIC1 | 2 | N/A |
| CD68 | Thermo Fisher (Waltham, MA) | MA5-13324 | Mouse anti-human (M) | KP1 | 2 | N/A |
| CD163 | Spring Biosciences (Cambridge, MA) | E18682 | Rabbit anti-human (P) | N/A | UN | N/A |
| CD204 | Sigma-Aldrich (St. Louis, MO) | HPA000272 | Rabbit anti-human (P) | N/A | 2 | N/A |
| CD209 | Santa Cruz Biotechnology | sc-65740 | Mouse anti-human (M) | DC28 | 2 | N/A |
| HCLS1 | Sigma-Aldrich | HPA-019143 | Rabbit anti-human (P) | N/A | 5 | N/A |
| Ly6C | BioXCell | BE0203 | Rat anti-mouse (M) | Monts 1 | N/A | 5 |
| Ly6G | BioXCell | BE0075 | Rat anti-mouse (M) | RB6-8C5 | N/A | 5 |
| LYVE-1 | R&D Systems | AF2089 | Goat anti-human (P) | N/A | 5 | 5 |
| Lyve-1 | AngioBio (San Diego, CA) | 11-034 | Rabbit anti-mouse (P) | N/A | 5 | 5 |
| MD2 | Biodefense and Emerging Infections Research Resources Repository (Manassas, VA) | NR-3887 | Mouse anti-human MD2 | U54.M.hMD2.9.1 | 10 | N/A |
| Pan-cytokeratin | Santa Cruz Biotechnology | sc-15367 | Rabbit anti-mouse/human (P) | N/A | 2 | N/A |
| Podoplanin | R&D Systems | AF3670 | Sheep anti-human (P) | N/A | 5 | N/A |
| Podoplanin | BioXCell | BE0236 | Syrian hamster anti-mouse (M) | 8.1.1 | 10 | 5 |
| PROX1 | R&D Systems | AF2727 | Goat anti-human (P) | N/A | 5 | N/A |
| PU.1 | Sigma-Aldrich | HPA044653 | Rabbit anti-human (P) | N/A | 5 | N/A |
| TLR4 | Imgenex (San Diego, CA) | IMG-6307A | Rabbit anti-human (P) | N/A | 5 | N/A |
| VE-cadherin | Thermo Fisher | PA6-19612 | Rabbit anti-mouse/human (P) | N/A | 10 | N/A |
| VEGFR-3 | R&D Systems | AF349 | Goat anti-human (P) | N/A | 5 | 5 |
| Vegfr-3 | R&D Systems | AF743 | Goat anti-mouse (P) | N/A | N/A | 5 |
HCLS, hematopoietic cell–specific Lyn substrate-1; IF, immunofluorescence; LYVE-1, lymphatic vessel endothelial hyaluronan receptor 1; M, monoclonal IgG; MD2, myeloid differentiation 2; N/A, not applicable; P, polyclonal IgG; PROX1, prospero homeobox protein 1; TLR4, toll-like receptor 4; UN, unknown; VE, vascular endothelial; VEGFR-3, VE growth factor receptor 3.