Table 2.
List of qPCR Primers for Human and Mouse Genes
| Primer∗ | Product size, bp | Forward sequence | Reverse sequence |
|---|---|---|---|
| Human primers | |||
| ACTB† | 131 | 5′-TCCTCTCCCAAGTCCACACAGG-3′ | 5′-GGGCACGAAGGCTCATCATTC-3′ |
| COUPTFII | 129 | 5′-CGGGTGGTCGCCTTTATGG-3′ | 5′-ACAGGCATCTGAGGTGAACAG-3′ |
| ITGA9 | 89 | 5′-GACGCTGATCCCTTGCTATGA-3′ | 5′-CGGTGAAGAAGCCCGCTATC-3′ |
| LYVE1 | 150 | 5′-TGGGGATCACCCTTGTGAG-3′ | 5′-AGCCATAGCTGCAAGTTTCAAA-3′ |
| PDPN | 128 | 5′-AGAGCAACAACTCAACGGGA-3′ | 5′-TGTAGTCTCAGTGTCATCTTC-3′ |
| PROX1 | 130 | 5′-GGATGTTGAGTATTCAGTGGTGC-3′ | 5′-CTGGGAAATTATGGTTGCTCCT-3′ |
| TIE2 | 105 | 5′-TTAGCCAGCTTAGTTCTCTGTGG-3′ | 5′-AGCATCAGATACAAGAGGTAGGG-3′ |
| VEGFC† | 791 | 5′-ACTCTTCCCCAGCCAATGTG-3′ | 5′-ATCCTGGCTCACAAGCCTTC-3′ |
| VEGFR3 | 259 | 5′-GCACTGCCACAAGAAGTACCT-3′ | 5′-GCTGCACAGATAGCGTCCC-3′ |
| Mouse primers | |||
| Actb† | 153 | 5′-GGCTGTATTCCCCTCCATCG-3′ | 5′-CCAGTTGGTAACAATGCCATGT-3′ |
| Cd34 | 157 | 5′-AAGGCTGGGTGAAGACCCTTA-3′ | 5′-TGAATGGCCGTTTCTGGAAGT-3′ |
| CoupTFII | 108 | 5′-TTCACCCATGTCAGCCGAC-3′ | 5′-GGCCTTGAGGCAGCTATACTC-3′ |
| Itga9 | 208 | 5′-AAGTGTCGTGTCCATACCAAC-3′ | 5′-GGTCTGCTTCGTAGTAGATGTTC-3′ |
| Lyve1 | 112 | 5′-CAGCACACTAGCCTGGTGTTA-3′ | 5′-CGCCCATGATTCTGCATGTAGA-3′ |
| Nrp2 | 67 | 5′-GCTGGCTACATCACTTCCCC-3′ | 5′-CAATCCACTCACAGTTCTGGTG-3′ |
| Pdpn | 159 | 5′-ACCGTGCCAGTGTTGTTCTG-3′ | 5′-AGCACCTGTGGTTGTTATTTTGT-3′ |
| Prox1 | 138 | 5′-GTGGTGCAACACGCAGATG-3′ | 5′-TGCCACCGTTTTTGTTCATGT-3′ |
| Tlr4 | 129 | 5′-ATGGCATGGCTTACACCACC-3′ | 5′-GAGGCCAATTTTGTCTCCACA-3′ |
| Tlr9 | 118 | 5′-ATGGTTCTCCGTCGAAGGACT-3′ | 5′-GAGGCTTCAGCTCACAGGG-3′ |
| Vegfc† | 160 | 5′-GAGGTCAAGGCTTTTGAAGGC-3′ | 5′-CTGTCCTGGTATTGAGGGTGG-3′ |
| Vegfr2 | 133 | 5′-TTTGGCAAATACAACCCTTCAGA-3′ | 5′-GCAGAAGATACTGTCACCACC-3′ |
| Vegfr3 | 182 | 5′-CGGGCTACCTGTCCATCATC-3′ | 5′-TGTCACAGCTGCTGCCTTTA-3′ |
COUPTFII, chicken ovalbumin upstream promoter transcription factor 2; ITGA9, integrin subunit alpha 9; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; Nrp, neuropilin; PDPN, podoplanin; qPCR, real-time quantitative PCR; Tie2, angiopoietin-1 receptor; Tlr, toll-like receptor; VEGFR, vascular endothelial growth factor receptor.
∗
Primers were designed based on human or mouse coding sequence of targets found in the National Center for Biotechnology Information database. All primers were validated using human or mouse universal cDNA. Primers were confirmed to exclusively detect species-specific cDNA.
†
Primers that were used to design TaqMan probes for absolute transcript copy number assay.