Table 3.

Normalized C_T Values of Lymphatic-Specific Genes in Pdpn-Positive and Pdpn-Negative TAMs

Gene	All CD11b+	PDPN+	PDPN−	Fold change		P value∗
				PDPN+ versus all CD11b+	PDPN− versus all CD11b+
Pdpn	13.45 ± 0.01	8.74 ± 0.42	21.41 ± 0.10	21.27 ± 5.55	−250 ± 0.002	0.062
Vegfr3	16.12 ± 0.24	13.30 ± 0.22	21.31 ± 0.25	7.10 ± 0.76	−33.3 ± 0.003	0.011
Vegfc	15.96 ± 0.19	13.41 ± 0.14	15.04 ± 0.35	5.87 ± 0.41	1.93 ± 0.32	0.017
Lyve1	17.69 ± 0.23	14.90 ± 0.27	16.37 ± 0.13	6.98 ± 0.91	2.50 ± 0.16	0.04
Prox1	18.26 ± 0.01	13.92 ± 0.23	21.53 ± 0.07	20.38 ± 2.25	−9.09 ± 0.004	0.012
Nrp2	12.08 ± 0.22	8.63 ± 0.23	10.52 ± 0.23	11.04 ± 1.26	2.97 ± 0.26	0.024
Itga9	15.43 ± 0.11	7.99 ± 0.02	16.91 ± 0.39	173.65 ± 1.81	−2.74 ± 0.07	0.0001
Vegfr2	14.58 ± 0.05	9.96 ± 0.26	16.85 ± 0.01	24.87 ± 3.17	−4.76 ± 0.001	0.02
Couptf2	14.20 ± 0.16	12.59 ± 0.05	13.93 ± 0.42	3.06 ± 0.74	1.23 ± 0.25	0.016

Data are expressed as means ± SEM.

PDPN, podoplanin; TAM, tumor-associated macrophage.

^∗P values represent differences in gene expression between Pdpn-positive and Pdpn-negative CD11b⁺ macrophages.