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. 2019 Dec 3;12:279. doi: 10.1186/s13068-019-1617-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Time-course analysis of xylan hydrolysis by the truncation mutants. a–c Xylan hydrolysis by TM1 (a), TM1 + TM2 (b), and TM3 (c). d Hydrolysis of xylotetraose by CbCel48B was inhibited by increasing amounts of xylobiose. The hydrolysis was carried out at 75 °C in the McIlvaine buffer (pH 6.5) for 18 h and at different time intervals the samples were taken out for HPAEC–PAC analysis