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. 2019 Oct;189(10):1986–2001. doi: 10.1016/j.ajpath.2019.07.003

Figure 4.

Figure 4

A and B: Bone marrow–derived macrophages (BMMs; A) and Kupffer cells (B) were treated with necrotic hepatocytes, 100 ng/mL high mobility group box 1 (HMGB1), or 100 nmol/L plasmin for 6 hours. Significantly different from control cells. C: BMMs were treated with the indicated concentrations of HMGB1. Tumor necrosis factor (TNF)-α mRNA was measured by real-time PCR. D–F: BMMs were treated with plasmin in the presence or absence of HMGB1 for 6 hours. Cytokine mRNAs and chemokine (C-C motif) ligand (Ccl2) protein were quantified. G–I: BMMs were treated with plasmin, followed by treatment with necrotic hepatocytes from wild-type (WT) or hepatocyte-specific HMGB1 knockout mice. J: BMMs were treated with TPCA1 or dimethyl sulfoxide (DMSO) for 30 minutes, followed by treatment with plasmin with and without HMGB1. TNF-α mRNA was measured. Significantly different from cells treated with TPCA1. Data are expressed as means ± SEM (A–J). n = 3 (A–J). *P < 0.05 versus control (A and B); †P < 0.05 versus vehicle (C–J). ND, not detected.