Figure 9.

Mice were treated with 300 mg/kg acetaminophen (APAP), followed by treatment with tranexamic acid (TA), as detailed in Materials and Methods. After 72 hours, the livers were digested and CD45, CD68, and F4/80 were detected in the nonparenchymal cell fraction by flow cytometry. A: The gating strategy used for flow cytometry. B and C: The intensity of F4/80 staining within the CD45⁺CD68⁺ population was quantified. D and E: The percentage of F4/80^low and F4/80^hi macrophages within the CD45⁺CD68⁺ population was quantified. Data are expressed as means ± SEM (C–E). n = 3 mice per group (C–E). *P < 0.05 versus APAP + vehicle.