REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies (anti-)
Myc tag	Millipore	cat# 05-724; RRID: AB_309938
ERK2	Santa Cruz biotechnology	cat# sc-154; RRID: AB_2141292
Flag-Tag	Sigma-Aldrich	cat# 3165; RRID: AB_259529
LAMP2 (aka CD107b)	BD PharMingen	cat# 555803; RRID: AB_396137
SLC3A2 (aka CD98hc)	Santa Cruz biotechnology	cat# sc-9160; RRID: AB_638288
SLC1A5 (aka ASCT2)	Cell Signaling	cat# 5345; RRID: AB_10621427
SLC7A5 (aka LAT1)	Cell Signaling	cat# 5347; RRID: AB_10695104
LC3B	Cell Signaling	cat# 2775; RRID: AB_515950
Phospho S6 Ribosomal protein	Cell Signaling	cat# 4858; RRID: AB_516156
S6 Ribosomal protein	Cell Signaling	cat# 2317; RRID: AB_2238583
Phospho 4E-BP1	Cell Signaling	cat# 2855; RRID: AB_560835
4E-BP1	Cell Signaling	cat# 9644; RRID: AB_2097841
Atg7	Cell Signaling	cat# 8558; RRID: AB_10831194
Phospho AKT (S473)	Cell Signaling	cat# 9271; RRID: AB_329825
Phospho AKT (T308)	Cell Signaling	cat# 4056; RRID: AB_331163
AKT	Cell Signaling	cat# 9272; RRID: AB_329827
PPARγ	Cell Signaling	cat# 2430; RRID: AB_823599
Anti-mouse IgG HRP-linked	Cell Signaling	cat# 7076S; RRID: AB_330924
Anti-rabbit IgG HRP-linked	Cell Signaling	cat# 7074S; RRID: AB_2099233
Phospho-IRS-1	Cell Signaling	cat# 2388; RRIS: AB_330339
Phospho p70 S6 Kinase	Cell Signaling	cat# 9234; RRID: AB_2269804
p70 S6 Kinase	Cell Signaling	cat# 2708; RRID: AB_390722
ARF6	Cell Signaling	cat# 3546; RRID: AB_2058484
V5-Tag	Cell Signaling	cat# 13202; RRID: AB_2687461
LAMP2 Alexa Fluor 488 conjugate	Thermo Fisher Scientific	MA1-205-A488; RRID: AB_2688309
Myc Tag Alexa Fluor 488	Millipore	cat#16-224; RRID: AB_442399
Scamp3	Proteintech	26888-1-AP; RRID: AB_2810962
V5-Tag DyLight 650 conjugate	Thermo Fisher Scientific	MA5-15253-D650; RRID: AB_2537642
VPS35	Abcam	ab97545; RRID: AB_10696107
Chemicals, Peptides, and Recombinant Proteins
D-Phenylalanine	Sigma-Aldrich	cat# P1751
L-Leucine methyl ester hydrochloride	Sigma-Aldrich	cat# L1002
L-γ-glutamyl-p-nitoanilide	Sigma-Aldrich	cat# G6133
L-Leucine	Sigma-Aldrich	cat# L8000
cycloheximide	Sigma-Aldrich	cat# C4859
puromycin	Sigma-Aldrich	cat# P9620
polybrene	Sigma-Aldrich	cat# H9268
doxycyclin	Sigma-Aldrich	cat# D9891
Protein A Sepharose beads	Sigma-Aldrich	cat# P9424
Anti-FLAG M2 Magnetic Beads	Sigma-Aldrich	cat# M8823
bicinchonic acid solution	Sigma-Aldrich	cat# B9643
Copper (II) sulfate solution	Sigma-Aldrich	cat# C2284
troglitazone	Sigma-Aldrich	cat# T2573
insulin	Sigma-Aldrich	cat# I9278
IBMX	Sigma-Aldrich	cat# I5879
dexamethasone	Sigma-Aldrich	cat# D4902
Oil Red O solution	Sigma-Aldrich	cat# O1391
Earle’s Balanced Salt Solution	Sigma-Aldrich	cat# E2888
Fetal Bovin Serum	Thermo Fisher Scientific	cat# 10270-106
penicillin/streptomycin	Thermo Fisher Scientific	cat# 15140-122
glutamine	Thermo Fisher Scientific	cat# 25030-024
accutase	Thermo Fisher Scientific	cat# A11105-01
trypsin	Thermo Fisher Scientific	cat# 15090-046
Dulbecco’s Modified Eagle Medium	Thermo Fisher Scientific	cat# 21969-035
50X MEM amino acid solution	Thermo Fisher Scientific	cat# 11130-036
LysoTracker Deep Red	Thermo Fisher Scientific	cat# L12492
HALT protease and phosphatase inhibitor cocktail	Thermo Fisher Scientific	cat# 78442
Rapamycin	LC laboratories	cat# R-5000
Bafilomycin A1	LC laboratories	cat# B-1080
Deposited Data
Full scans of western blot data and original microscopy images	This paper	Mendeley Data: https://doi.org/10.17632/37tgjx44g4.1
Experimental Models: Cell Lines
Saos2 Tet-On DRAM1-myc tag	This lab	Crighton et al. 2006
Phoenix-AMPHO	ATCC	cat# CRL-3213
HEK293T	ATCC	cat# CRL-3216
3T3-L1	ATCC	cat# CL-173
DRAM1 fl/fl MEF	This article	N/A
ATG7 fl/fl MEF	This article	N/A
ATG7 fl/fl DRAM1 KO MEF	This article	N/A
HeLa	Beatson Institute stock (obtained from ATCC)	N/A
Experimental Models: Organisms/Strains
C57BL/6 mice	Charles River	Strain Code: 027
DRAM1fl/fl C57BL/6 mice	This article	N/A
DRAM1+/− C57BL/6 mice	This article	N/A
ATG7fl/fl C57BL/6 mice	Komatsu et al. 2005	Komatsu et al. 2005
Oligonucleotides
See table for primer sequences		N/A
Recombinant DNA
pBabe-puro	Addgene	cat# 1764
pLenti-puro	Addgene	cat# 39481
pLX304-SLC3A2	DNASU plasmid repository	cat# HsCD00440549
pLX304-SLC1A5	DNASU plasmid repository	cat# HsCD00436374
pLZRS-SLC3A2-RFP-GFP	This article	N/A
pLZRS- RFP-GFP-SLC3A2	This article	N/A
psPAX2	Addgene	cat# 12260
pMD2.G	Addgene	cat# 12259
p LAMP1-mRFP-Flag2X	Addgene	cat# 34611
pCMV6-mouse-DRAM1	Origene	cat# MR220640
pLentiCRISPR v2	Addgene	cat# 52961
pLentiCRISPR v2 mouse DRAM1 #A	This article	N/A
pLentiCRISPR v2 mouse DRAM1 #B	This article	N/A
pLentiCRISPR v2 NTC1	This article	N/A
pLentiCRISPR v2 NTC2	This article	N/A
si/shRNA
Renilla shRNA (Non-targeting control)	Mirimus	LPE shRen.713
SLC1A5 shRNA	Mirimus	cat# SLC1A5_798
SLC1A5 shRNA	Mirimus	cat# SLC1A5_1062
SLC7A5 shRNA	Mirimus	cat# SLC7A5_1857
SLC7A5 shRNA	Mirimus	cat# SLC7A5_2000
ON-TARGETplus SLC1A5 smartpool siRNA	GE Dharmacon	cat# L-007429-00-0005
ON-TARGETplus SLC7A5 smartpool siRNA	GE Dharmacon	cat# L-041166-01-0005
ON-TARGETplus non-targeting controls siRNA	GE Dharmacon	cat# D-001810-01-05, D-001810-02-05, D-001810-03-05, D-001810-04-05
Software and Algorithms
ImageJ64	NIH	https://imagej.nih.gov/ij/
Optimized CRISPR Design	Feng Zhang Lab (MIT)	http://zlab.bio/guide-design-resources
ZEN 2012 software	Zeiss	N/A
FlowJo software v.7.6.5	FlowJo	N/A