Fig. 1.

Levels of synaptic marker proteins in hippocampus and temporal lobe in AMC and RISE rats at different stages of epileptogenesis.

RISE rats were sacrificed 24 h (SE), 2–4 weeks (LP) or ~3+ months (SRS) post pilocarpine injection and hippocampi and temporal lobes dissected, immediately flash frozen in liquid nitrogen and stored at −80 °C until use. Samples were homogenised, protein matched and 40 μg of total protein was loaded per lane.

Left panels: Representative Western blots of hippocampal and temporal lobe samples from 4 (SE) or 5 (LP and SRS) separate AMC and RISE rats immunoblotted for synaptophysin, PSD95 and gephyrin. β-tubulin III and β-actin were used as loading controls.

Right panels: Quantification of immunoreactive bands normalised to their respective loading controls. Error bars represent the mean ± SD from 4 samples, with the mean of the control group set to 1. *P < 0.05 vs Ctrl, **P < 0.01 vs Ctrl, ***P < 0.001 vs Ctrl (Unpaired t-test).