Fig. 2.

Effect of staurosporine and WNK463 on KCC activity in red cells from patients with SCA. Red cells (20% Hct) were pre-incubated in N-MBS sequentially for two periods of 30 min in the presence of vehicle (DMSO) or drug (WNK463 40 nM or staurosporine 100 μM), as indicated. They were then equilibrated in Eschweiler tonometers for 20 min at 150 mmHg in the continued presence of WNK463 and/or staurosporine and KCC activity measured as described in the legend to Fig. 1. Histograms represent means ± SEM, n = 4. * p < 0.05, ** p < 0.01 compared to red cells incubated in the absence of WNK463; ^# p < 0.05 between groups as indicated