Fig. 3.

Effect of N-ethylmaleimide (NEM) and WNK463 on KCC activity in red cells from patients with SCA. Red cells (20% Hct) were pre-incubated in N-MBS sequentially for two periods of 30 min in the presence of vehicle (DMSO) or drug (WNK463 40 nM or NEM 1 mM), as indicated. They were then equilibrated in Eschweiler tonometers for 20 min at 150 mmHg in the continued presence of WNK463 and/or NEM and KCC activity measured as described in the legend to Fig. 1. Histograms represent means ± SEM, n = 4. * p < 0.05, ** p < 0.01 compared to red cells incubated in the absence of WNK463; ^# p < 0.05 between groups as indicated