Fig. 6.

Effect WNK463 on oxygen-dependent KCC activity in red cells from patients with SCA. Red cells (20% Hct) were pre-incubated in N-MBS in the presence or absence of WNK463 (40 nM) at 37 °C in air. They were then equilibrated in Eschweiler tonometers for 20 min at 0–150 mmHg, after which aliquots were diluted tenfold into flux tubes, all in the continued presence or absence of WNK463, and KCC activity was measured as described in the legend to Fig. 1. Symbols represent means ± SEM (error bars are smaller than symbols), n = 6