Fig. 7.

Effect of SPAK/OSR1 inhibitors on KCC activity in HbAA RBCs. Red cells (40% Hct) were pre-incubated in N-MBS in the presence of 0–100 μM STOCK2S-26016 or 0–2 mM HK01 at 37 °C in air. They were then equilibrated in Eschweiler tonometers for 20 min at 0–150 mmHg, after which KCC activity was measured as described in the legend to Fig. 1. a Effect of STOCK2S-26016 on KCC activity in HbAA cells, n = 3. b Effect of HK01 on KCC activity in HbAA cells, n = 3. Histograms represent means ± SEM of n individual samples. * p < 0.05