Fig. 5.

M4P improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit IgG in rat brains. The experimental protocol is similar as in Fig. 4b. Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b, statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c, statistical analysis was performed by two-tailed unpaired Student’s t test. *p < 0.05