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. 2019 Apr;60(5):1604–1613. doi: 10.1167/iovs.19-26553

Figure 4.

Figure 4

Inhibition of UCP2 increases Glut1 and VEGF-induced glucose uptake in hRMVECs. Western blot of Glut1 and UCP2 (A, B) in hRMVECs treated with control or genipin (10 μM) for 2 hours and (C, D) in hRMVECs transfected with UCP2 siRNA or control siRNA. (A, C) Representative gel images. (B, D) Quantification of densitometry of Glut1). (E) Glucose uptake measured by (N-[7-Nitrobenz-2-oxa-1,3-diazol-4-yl]Amino)-2-Deoxyglucose and (F) ATP production in hRMVECs treated with genipin (10 μM) or control for 30 minutes (*P < 0.05, **P < 0.01, ***P < 0.001 versus. Control; †P < 0.05 versus Control siRNA; n = 4 for Western blots and n = 9 for glucose uptake and ATP production).