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. Author manuscript; available in PMC: 2019 Dec 4.
Published in final edited form as: Mol Cell. 2019 Jan 24;73(5):885–899.e6. doi: 10.1016/j.molcel.2018.12.008

Figure 2. BRCA2 Synthetic Lethal Screen Results.

Figure 2.

(A-C) Volcano plots for the shRNA colonic, CRISPR colonic, and CRISPR ovarian B2SL screens. Significance (-log10FDR) is plotted against the genetic interaction (GI) score (average log2 fold-change for each gene in BRCA2 MUT versus BRCA2 WT cells). Genes that met a significance threshold of -log10FDR>1 are color-coded as green for relative dropout or red for relative enrichment in BRCA2 MUT vs BRCA2 WT cells. (D-F) Results from the colonic shRNA, colonic CRISPR, and ovarian CRISPR B2SL screens, plotted as the log2 fold-change in BRCA2 MUT cells against the log2 fold-change in BRCA2 WT cells. (G-H) Comparison of GI score (average log2 fold-change for each gene in BRCA2 MUT versus BRCA2 WT cells) in the ovarian CRISPR screen versus the colonic CRISPR screen (G), and the ovarian CRISPR screen versus primary shRNA screen (H).