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. Author manuscript; available in PMC: 2019 Dec 4.
Published in final edited form as: Mol Cell. 2019 Jan 24;73(5):885–899.e6. doi: 10.1016/j.molcel.2018.12.008

Figure 5. FEN1 and APEX2 loss leads to DNA damage in BRCA2 mutant cells.

Figure 5.

(A) Immunofluorescence was performed on ovarian B2MUT cells transfected with the indicated siRNAs, using an antibody to γH2AX, to evaluate foci formation. (B) Quantification of the number γH2AX foci formed in (G) in 30 representative cells transfected with siRNA to the indicated genes. (C) Examination of DDR signaling in extracts of WT and BRCA2 mutant cells transfected with the indicated siRNAs and immunoblotted with the indicated antibodies. (D) Quantitation of phospho-Chk1, phospho-H2AX and phospho-RPA32 from the BRCA2 mutant cell extracts in experiment (C).