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. 2019 Nov 27;2(6):e201900453. doi: 10.26508/lsa.201900453

Figure 5. Young Cx3cr1-deficient mice display a premature aging phenotype.

Figure 5.

Microglia were isolated from 2-mo-, 1-yr-, or 2-yr-old Cx3cr1+/+ (WT), Cx3cr1+/eGFP (Het), or Cx3cr1eGFP/eGFP (KO) mice and used for RNA-seq analysis, followed by quantification and unsupervised hierarchical clustering for the top 100 DEGs (KO versus WT, by P-value) at each time point, resulting in a combined list of 254 genes. In 2-mo-old mice, the samples separate by genotype, whereas in older mice, they first separate by age and then genotype. Overall, 2-mo-old Cx3cr1-Het and KO microglia resemble aged mice of any genotype. Some genes increase with both age and Cx3cr1 deletion and show the highest expression in aged Cx3cr1-KO microglia.