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Quantification and unsupervised hierarchical clustering for microglia from 2-yr-old Cx3cr1+/+ (WT, n = 9), Cx3cr1+/eGFP (Het, n = 8), or Cx3cr1eGFP/eGFP (KO, n = 11) mice highlights a strong effect of gender on gene expression in old mice. The samples primarily separate by gender and not Cx3cr1 genotype. Selected genes and associated pathways (manual annotation) are represented in different colors.
