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. 2019 Nov 27;2(6):e201900453. doi: 10.26508/lsa.201900453

Figure S1. qRT-PCR analysis of the expression of selected DEGs identified by RNA-seq.

Figure S1.

Microglia were isolated from 2-mo-old Cx3cr1+/+ (WT, n = 4), Cx3cr1+/eGFP (Het, n = 4), or Cx3cr1eGFP/eGFP (KO, n = 4) mice. The RNA isolated from microglia of each mouse and used for qRT-PCR analysis. (A) Selected genes that show changes in expression after loss of Cx3cr1 by RNA-seq and confirmed by qRT-PCR. (B) Examples of genes that are not modulated in microglia by Cx3cr1 genotype. Egr1 was identified as a DEG, but Trem2 was not differentially expressed.