Figure S3. Loss of Cx3cr1 does not affect microglial transcriptional response to LPS.
2-mo-old Cx3cr1+/+ (WT, n = 7), Cx3cr1+/eGFP (Het, n = 6), or Cx3cr1eGFP/eGFP (KO, n = 6) mice were injected with 1 mg/kg LPS IP (or saline control, four WT and three KO animals only). Twenty-four hours later, microglia were sorted from isolated brains and subjected to RNA-seq analysis. Unsupervised cluster analysis of microglial of top 200 DEGs (saline versus LPS comparison) shows a strong effect of LPS, but no clear separation of the samples by genotype after LPS treatment.