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. 2019 Nov 27;2(6):e201900453. doi: 10.26508/lsa.201900453

Figure S4. Unsupervised hierarchical clustering by age shows that similar pathways are modulated by aging as by Cx3cr1 loss.

Figure S4.

Microglia were isolated from 2-mo-, 1-yr-, or 2-yr-old Cx3cr1+/+ (WT), Cx3cr1+/eGFP (Het), or Cx3cr1eGFP/eGFP (KO) mice and used for RNA-seq analysis, followed by quantification and unsupervised hierarchical clustering for the top 200 DEGs (by P-value) between WT 2-yr- versus 2-mo-old mice. Aging alters the expression of genes in pathways modulated by Cx3cr1 deletion as well. Although separation by genotype is visible in microglia form 2-mo-old mice, it is lost in aged mice.