Table 1.
Expression of Tspan5, Tspan14, and Tspan15 in the three cell lines used in the study and consequence of the manipulation of Tspan5 and Tspan15 expression levels on ADAM10 surface expression and endocytosis.
| PC3 | U2OS | HeLa | |
|---|---|---|---|
| Surface expression in WT cell lines | |||
| ADAM10 | ++ | ++ | + |
| Tspan5 | + | + | 0 |
| Tspan15 | ++ | 0 | 0 |
| Tspan14 | 0 | ++ | + |
| ADAM10 surface expression | |||
| Tspan5 transfection | ↓ | = | ↑ |
| Tspan15 transfection | = | ↑↑ | ↑ |
| KO Tspan5 | = | = | NA |
| KO Tspan15 | ↓ | NA | NA |
| ADAM10 endocytosis | |||
| Tspan5 transfection | ↑ | ↑ | = |
| Tspan15 transfection | = | ↓ | ↓ |
| KO Tspan5 | = | ↓ | NA |
| KO Tspan15 | ↑ | NA | NA |
The expression level of Tspan14 is only determined at the RNA level. NA, not applicable.