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. 2019 Dec 4;17(12):e3000494. doi: 10.1371/journal.pbio.3000494

Table 2. Alignment details for the 31 gene fragments used in this study.

    M11 This study
Gene Region # sites # taxa # sites # taxa GC content Identical sites Matrix comp. Mean coverage
A2AB exon 848 165 (4) 603 290 (A) 61.0% 85.2% 91.9% 284.1
ADORA3 exon 332 163 (3) 369 429 (A) 45.8% 83.0% 86.6% 427.7
ADRB2 exon 803 155 (5) 846 218 (A) 53.3% 86.1% 94.3% 215.1
APOB exon 2,624 168 (5) 2,523 587 (A) 41.8% 35.2% 51.7% 334.7
APP NC 696 152 (0) 636 414 (M) 37.7% 84.1% 94.7% 403.6
ATP7 exon 686 163 (3) 723 469 (A) 40.6% 83.4% 91.5% 459.8
BCHE exon 995 149 (5) 1,020 309 (A) 40.8% 82.0% 94.0% 300.3
BDNF exon 560 157 (5) 612 508 (A) 55.1% 85.3% 87.8% 489.4
BMI1 NC 336 150 (0) 292 191 (O) 33.5% 92.4% 98.9% 189.9
BRCA1 exon 3,092 160 (0) 3,264 969 (M) 41.7% 36.1% 47.0% 538.1
BRCA2 exon 5,045 163 (0) 4,854 306 (O) 34.4% 41.8% 52.5% 183.3
CNR1 exon 1,004 162 (5) 1,017 356 (A) 54.0% 83.9% 93.8% 342.6
COI mtDNA -- -- 672 1,686 (A) 43.0% 76.6% 96.1% 1,657.4
CREM NC 441 155 (0) 350 331 (O) 44.7% 80.7% 95.8% 322.5
CYTB mtDNA -- -- 1,167 3,581 (A) 41.5% 65.8% 90.4% 3,318
DMP1 exon 1,361 161 (0) 1,503 415 (M) 51.6% 28.3% 41.1% 205.3
EDG1 exon 959 153 (5) 963 313 (A) 56.3% 84.1% 95.5% 301.8
ENAM exon 3,899 162 (0) 3,477 247 (O) 44.5% 58.2% 68.9% 215.5
FBN1 NC 745 150 (0) 669 301 (M) 33.6% 81.8% 93.6% 289.3
GHR exon 947 165 (4) 1,044 978 (A) 49.1% 58.2% 70.1% 794.5
IRBP exon 1,253 161 (5) 1,146 1,345 (A) 59.4% 69.5% 85.4% 1,231.9
ND1 mtDNA -- -- 975 962 (A) 40.6% 71.0% 94.4% 925.5
ND2 mtDNA -- -- 1,074 983 (A) 37.2% 57.7% 86.3% 873.3
PLCB4 NC 350 156 (0) 288 478 (M) 40.1% 75.9% 96.2% 469.6
PNOC exon 320 144 (0) 339 410 (M) 62.5% 79.7% 82.7% 403.5
RAG1a exon 1,799 168 (5) 1,050 639 (A) 51.5% 66.2% 84.6% 556.8
RAG1b exon -- -- 1,071 936 (A) 50.6% 61.8% 81.1% 763.6
RAG2 exon 446 163 (5) 450 889 (A) 44.1% 86.9% 97.5% 879.4
TTN exon 4,437 168 (5) 4,479 345 (A) 42.0% 41.1% 58.9% 205.4
TYR1 exon 428 151 (5) 429 336 (A) 46.9% 85.4% 98.8% 334.2
VWF exon 1,172 162 (3) 1,194 821 (A) 59.0% 64.4% 84.0% 707.5
Total concatenated 35,603 164 (5) 39,099 4,098 (A) 45.3% 13.4% 11.9% 1,651.2

Comparison is made to the family-level phylogenetic study of Meredith and colleagues [60] (M11) for the number of aligned sites (with gaps) and mammalian taxa sampled per gene. The per-gene out-group(s) are noted in parentheses, either as the number of out-group taxa used in M11 or identifying the out-group used in gene tree building for this study. Note that RAG1a and RAG1b fragments are combined in M11. Site identity is calculated pairwise, matrix comp. is the sites-by-taxa percentage of ungapped sites, and mean coverage refers to the mean ungapped sites per matrix column (i.e., mean taxon sampling per site).

Abbreviations: A, Anolis; comp., completeness; GC, guanine-cytosine; M, Monodelphis; M11, Meredith and colleagues 2011; mtDNA, mitochondrial DNA; NC, noncoding; O, Ornithorhynchus