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. 2019 Nov 20;15(11):e1008477. doi: 10.1371/journal.pgen.1008477

Fig 2. Desumoylation of CCAN is partially redundantly controlled by Ulp2’s SIM and CCR.

Fig 2

A) Anti-Smt3 Western blot to observe total sumoylated proteins via the Ulp1-C580S pull-down approach. Relative loading amounts are indicated at the top of each sample lane. 2B-2F) Protein A tagged Mcm21, Ame1, Okp1, Mcm16 and Mcm22 were detected using an anti-Protein A antibody and Western blot analysis. Approximately 150-fold more loading was used for the eluted samples compared to the input samples. Sumoylated species of these CCAN subunits were only detected in the purified sample, which appear as higher molecular weight bands compared to the corresponding un-sumoylated CCAN subunit.