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. 2019 Nov 20;15(11):e1008477. doi: 10.1371/journal.pgen.1008477

Fig 3. Binding between Ulp2’s CCR and the kinetochore requires the Ctf3-Mcm16-Mcm22 complex.

Fig 3

A) Illustration of the hierarchical order of CCAN assembly. Various sub-complexes of CCAN are distinguished in colored boxes, in which essential subunits are shown in bold. B) Pull-down assay to observe the binding between Mcm21 and Ulp2’s CCR. C) Effect of mcm16Δ and ctf19Δ on the binding between Mcm21 and Ulp2’s CCR. D) Effect of chl4Δ and cnn1Δ on the binding between Mcm21 and Ulp2’s CCR. E) Effect of chl4Δ and cnn1Δ on the binding between Mcm16 Ulp2’s CCR. F) Effect of mcm16Δ, mcm22Δ and mcm21Δ on the binding between Ctf3 and Ulp2’s CCR. G) Effect of mcm16Δ and ctf3Δ on the binding between Mcm22 and Ulp2’s CCR. H) Effect of mcm22Δ and ctf3Δ on the binding between Mcm16 and Ulp2’s CCR. TAF tag refers to 6xHIS-3xFLAG-ProteinA (see footnote in S12 Table).