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. 2019 Nov 20;15(11):e1008477. doi: 10.1371/journal.pgen.1008477

Fig 5. Mcm16 plays a dual role in regulating CCAN sumoylation.

Fig 5

A) Chromosome loss rates of WT, ulp2-SIM3A, ulp2-CCR3A, ulp2-SIM3ACCR3A, mcm16Δ, mcm16Δ ulp2-SIM3A, mcm16Δ ulp2-CCR3A and mcm16Δ ulp2-SIM3ACCR3A mutants, measured by quantitative mating (also see S8 Table). B) Growth of various ulp2 and mcm16Δ mutants, following the acute removal of the complementing ULP2 plasmid by 5-fluoroorotic acid (5-FOA). C) Quantitative MS to measure the effect of mcm16Δ on intracellular sumoylation in the ulp2Δ mutant (also see S9 Table). D-E) Western blot analysis to observe the effect of mcm16Δ on Mcm21 and Ame1 sumoylation in the ulp2Δ mutant, following the enrichment of sumoylated proteins via the Ulp1-C580S affinity resin. F) Western blot analysis to observe the effect of mcm16Δ on the sumoylation of Mcm21 in various ulp2 mutants following the enrichment of sumoylated proteins via Ulp1-C580S affinity resin.